Sequence of Gs-WT-HA

Gs-WT-HA, also known as "GSL". This is rat Gs-alpha with the HA tag. It is in a Sal I HindIII cassette of pcDNA Id. pcDNA Id has an altered polylinker so that sites are: Sal I, Pst I, Eco RV, Hind III, Xho I, Sph I, Nsi I, Xba I. Listed below is the sequence between the Sal I and Hind III.

gtcgacGGGCTGCAGGTCCGCAGCAGCAGCTCCCGCGGCTCCTGCTCTGCTCCGCCTCGGCCCCGGAGCGAGGGGCGGAG AGCCGCGCGCTTGCCTTAGTCCGAGCCGCCACCCTCCCCGCGTTCCCCGCTTTCCCGGCCCGCCCGAGGCCGCCCGCCCC GTCCCCGCCGCCCCGCAGCCCGGCCGCGCCCCGCCGCCGCCATGGGCTGCCTCGGCAACAGTAAGACCGAGGACCAGCGC AACGAGGAGAAGGCGCAGCGCGAGGCCAACAAAAAGATCGAGAAGCAGCTGCAGAAGGACAAGCAGGTCTACCGGGCCAC GCACCGCCTGCTGCTGCTGGGTGCTGGAGAGTCTGGCAAAAGCACCATTGTGAAGCAGATGAGGATCCTACATGTTAATG GGTTTAACGGAGAGGGCGGCGAAGAGGACGTGCCGGATTACGCGTCCAACAGCGATGGTGAGAAGGCCACCAAAGTGCAG GACATCAAAAACAACCTGAAGGAGGCCATTGAAACCATTGTGGCCGCCATGAGCAACCTGGTGCCCCCCGTGGAGCTGGC CAACCCTGAGAACCAGTTCAGAGTGGACTACATTCTGAGCGTGATGAACGTGCCAAACTTTGACTTCCCACCTGAATTCT ATGAGCATGCCAAGGCTCTGTGGGAGGATGAGGGAGTTCGTGCCTGCTACGAGCGCTCCAACGAGTACCAGCTGATCGAC TGTGCCCAGTACTTCCTGGACAAGATTGATGTGATCAAGCAGGCCGACTACGTGCCAAGTGACCAGGACCTGCTTCGCTG CCGCGTCCTGACCTCTGGAATCTTTGAGACCAAGTTCCAGGTGGACAAAGTCAACTTCCACATGTTCGATGTGGGCGGCC AGCGCGATGAACGCCGCAAGTGGATCCAGTGCTTCAATGATGTGACTGCCATCATCTTCGTGGTGGCCAGCAGCAGCTAC AACATGGTCATCCGGGAGGACAACCAGACCAACCGTCTGCAGGAGGCTCTGAACCTCTTCAAGAGCATCTGGAACAACAG ATGGCTGCGTACCATCTCTGTGATCCTCTTCCTCAACAAGCAAGATCTGCTTGCTGAGAAGGTCCTCGCTGGGAAATCGA AGATTGAGGACTACTTTCCAGAGTTCGCTCGCTACACCACTCCTGAGGATGCGACTCCCGAGCCCGGAGAGGACCCACGC GTGACCCGGGCCAAGTACTTCATCCGGGATGAGTTTCTGAGAATCAGCACTGCTAGTGGAGATGGACGTCACTACTGCTA CCCTCACTTTACCTGCGCCGTGGACACTGAGAACATCCGCCGTGTCTTCAACGACTGCCGTGACATCATCCAGCGCATGC ATCTTCGCCAATACGAGCTGCTCTAAGAAGGGAACGCCCAAATTTAATTCAGCCTTAAGCACAATTAATTAAGAGTGAAA CGCAATCGTACAAGCAGTTGATCACCCACCATAGGGCATGATCAACACCGCAACCTTTCCCTTTTCTCCCCAGTGATTCT GAAAACCCCCTCTTCCCTTCAGCTTGCTTAGATGTTCCAAATTTAGTAAGCTT

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