Back to Conklin Lab home page Doxycycline Administration in Water:
Make 100x doxycycline stock solution of 20 mg/ml of Doxycycline (Sigma)with water. (It takes about 2 minutes of shaking to get this into solution). This solutioncan be frozen in 5 ml aliquots, wrapped in tin foil.
In mouse facility, add the 100X stock to water bottles for final concentration200 mcg/ml. It is preferable to use amber water bottles since tetracycline and doxycyclineare light sensitive (we use Wheaton "900" RediPak Amber GlassPackers with Caps, 250 ml, Fisher cat # 02-911-4)
It is not necessary to put sucrose in the water. For most purposes, thisdose of doxycycline in great excess of what is required to suppress gene expression in peripheral organs (higher doses may be needed to reach thebrain). Therefore we add fresh doxycycline-containing water weekly, butmore frequent water changes are unnecessary.
For IP injection of Doxycycline:
10 mcg of Doxycycline in 0.5 cc of water can be given as a single IP injection to a 30 gram mouse. This is only necessary for very sick mice.
For administration of Doxycycline in food pellets:
Doxycycline in food pellets are custom made by Bio-Serv (908-996-2155)One 8th Street, Frenchtown, NJ 08825. These pellets are made with 200 mgof doxycycline per kg of regular mouse chow and the addition of green foodcoloring. You need to FedEx the doxycycline to Bio-Serv, and radiation sterilizationof food pellets is needed if you have a "barrier" animal facility.When placing order, refer to catalog # "S3431, Gladstone", forthe exact specification of the chow. We order 100 kg at a time ($1366) andthis supplies us for 3-5 months.
We have found that doxycycline administration in the food requires lesswork than doxycycline in the water, since the animal care staff can replenishthe food. We find that the green food coloring is critical for keeping trackof which mice are on doxycyline.
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