Transgenic Mice
Conklin Lab
Transgenic Mice
The
Conklin Lab maintains several transgenic lines that utilize the tetracyclinetransactivator
system. These lines will be made available on a collaborative basis and as our
limited resources allow. (Jackson Lab should have manyof these lines soon).
Genotyping
mice
Mice
can be genotyped by PCR. After digesting a small piece of mousetail with proteinase
K, a small alliquot of sample which contains genomic DNA is analyzed by PCR
using transgene specific primers. Full
protocol available.
All oligonucleotide
sequences are shown as they should be ordered.
Tet-Responsive
Lines (tetO)
| Transgene:
tetO-Lac Z |
| 5´
primer: |
GGC GTG TAC GGT GGG
AGG |
| 3´
primer: |
GTT GGG AAG GGC GAT
CGG |
| PCR Fragment
Size: |
421 bp |
-
| Transgene:
tetO-G-alpha 12 |
| 5´
primer: |
CGA GAG CGG CAA GTC
CAC |
| 3´
primer: |
GCA GGC TCA GTC GGC
ATG TAC |
| PCR
Fragment Size: |
245
bp
|
| Transgene:
tetO-G-alpha i2 |
| 5´
primer: |
- GCA GAT CGA CTT
TGC TGA CC
|
| 3´
primer: |
TCT GTC GGC
ATG TAC TCC TC |
- PCR Fragment
Size:
|
240
bp |
| Transgene:
tetO-G-alpha q |
- 5´
primer:
|
TTG ACG TCC CCG ACT ACG
CG |
| 3´ primer: |
-
-
- CTT TGG CCC CCT
ACA TCG AC
|
| PCR Fragment
Size: |
- 258 bp
|
| Transgene:
tetO-Ro1/Ro2 |
| 5´ primer (Go148): |
GTT GGG AAG GGC GAT CGG |
| 3´ primer (Go147): |
GGC GTC TAC GGT GGG AGG |
| PCR Fragment
Size: |
178 bp |
Transactivator Lines
| Transgene:
tTA |
| 5´
primer: |
GCT GCT TAA
TGA GGT CGG |
| 3´
primer: |
CTC TGC ACC
TTG GTG ATC |
| PCR
Fragment Size: |
507
bp |
| Transgene:
MH-tTA |
| 5´
primer: |
TCA GAC CGA
GAT TTC TTC ATC CC |
| 3´
primer: |
CTC TGC ACC
TTG GTG ATC |
| PCR
Fragment Size: |
~1,000
bp |
| Transgene:
MMTV-tTA |
| 5´ primer: |
CTG ATC TGA GCT CTG AGT
G |
| 3´ primer:
|
CTC TGC ACC TTG GTG ATC
|
| PCR Fragment
Size: |
- 595 bp
|
Back to Conklin Lab home page
Protocols for genotyping the following mice can be found on the
Protocol's Page:
TetO-Rs1 mice
ColI(2.3)-tTA mice
NKX2-5-EmGFP (RP11-88L12 NKX2-5 EmGFP reporter mice)
