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Vantage DiVa Sorter
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We have the capability to support the following analysis and sorting applications:
- Sorting and analysis of live human HIV infected samples.
- Sorting and analysis of live human Stem Cell Lines (both Federally approved and not).
- Sorting under (relatively) sterile conditions with chilling provided for the sample and collected cells.
- Sorting (up to 10 colors) and analysis (currently up to 12 colors) of multicolor immunophenotyped samples.
- Sorting and analysis of CFP, GFP, YFP, mRFP, mTomato, and mCherry gene expression proteins.
- Calcium flux measurements using Indo-1.
- DNA analysis using standard dyes (Hoeschst, DAPI, PI, 7-AAD, etc.).
- Sorting stem cell side populations using Hoechst or DyeCycle Violet.
- Single cell sorting (cloning) into 96-well plates for culture and Ampligrid slides for single cell PCR.
- Sorting up to 4 populations at once into Eppendorf or 5 ml tubes.
- Analysis samples can be run directly from 96-well plates.
- Consulting on experimental design, and effective flow cytometry components for grants.
For more information please contact the Core Director.
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