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Supporting Gladstone
Vantage DiVa Sorter

We have the capability to support the following analysis and sorting applications:

  • Sorting and analysis of live human HIV infected samples.
  • Sorting and analysis of live human Stem Cell Lines (both Federally approved and not).
  • Sorting under (relatively) sterile conditions with chilling provided for the sample and collected cells.
  • Sorting (up to 10 colors) and analysis (currently up to 12 colors) of multicolor immunophenotyped samples.
  • Sorting and analysis of CFP, GFP, YFP, mRFP, mTomato, and mCherry gene expression proteins.
  • Calcium flux measurements using Indo-1.
  • DNA analysis using standard dyes (Hoeschst, DAPI, PI, 7-AAD, etc.).
  • Sorting stem cell side populations using Hoechst or DyeCycle Violet.
  • Single cell sorting (cloning) into 96-well plates for culture and Ampligrid slides for single cell PCR.
  • Sorting up to 4 populations at once into Eppendorf or 5 ml tubes.
  • Analysis samples can be run directly from 96-well plates.
  • Consulting on experimental design, and effective flow cytometry components for grants.

For more information please contact the Core Director.



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