Exiqon miRNA Arrays
The Genomics Core currently supports miRNA arrays made by Exiqon. These arrays use Locked Nucleic Acid (below) technology which provides a greater ability to distinguish separate perfectly matched hybrids from mismatches. The most commonly used Exiqon array is v11 of their mouse, mouse and rat array which contains more than 1700 capture probes, covering all miRNAs annotated in miRBase 11.0, as well as all viral miRNAs related to these species. In addition, this array contains capture probes for 428 new miRPlus™ human miRNAs that are proprietary to Exiqon. Information about miRPlus sequences can be readily obtained upon completion of a nondisclosure agreement. All probes are printed in quadruplicate on each array. We currently require a minimum of 300 ng total RNA for use with these arrays. If you would need to use smaller amounts of starting material, please contact us. While these arrays originally required 2 color hybridizations and by extension study designs, they now can also be used in single color study designs. Also see:
Exiqon*
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