The Gladstone Flow Cytometry Core offers state-of-the-art analysis and cell sorting, including analysis and isolation of live HIV-infected cells. The Core maintains the following instruments at the Gladstone Building at Mission Bay: two bench top analyzers (4 color and 12 color) and one sorter (10 color). These resources are equally available to all Gladstone researchers, researchers within the San Francisco General Hospital and UCSF communities, and other academic and commercial entities.
We have the capability to support the following analysis and sorting applications:
- Sorting and analysis of live human HIV infected samples.
- Sorting and analysis of live human Stem Cell Lines (both Federally approved and not).
- Sorting under (relatively) sterile conditions with chilling provided for the sample and collected cells.
- Sorting (up to 10 colors) and analysis (currently up to 12 colors) of multicolor immunophenotyped samples.
- Sorting and analysis of CFP, GFP, YFP, mRFP, mTomato, and mCherry gene expression proteins.
- Calcium flux measurements using Indo-1.
- DNA analysis using standard dyes (Hoeschst, DAPI, PI, 7-AAD, etc.).
- Sorting stem cell side populations using Hoechst or DyeCycle Violet.
- Single cell sorting (cloning) into 96-well plates for culture and Ampligrid slides for single cell PCR.
- Sorting up to 4 populations at once into Eppendorf or 5 ml tubes.
- Analysis samples can be run directly from 96-well plates.
- Consulting on experimental design, and effective flow cytometry components for grants.
For more information please contact the Core Manager..
Updated 07/03/08
