When performing expression analysis the tools available have typically included DNA microarrays which can screen thousands of transcripts in an unbiased manner at the expense of high cost per sample and measurement of gene expression relative to a standard sample. Alternatively, users have relied on qRT-PCR to accurately measure the expression levels of small numbers of transcripts. There has been an ongoing need for ways to measure larger numbers of transcripts than by traditional qRT-PCR at a lower cost per sample than with DNA microarrays.

The Genomics Core has recently begun to support OpenArrays from BioTrove which allow the screening of up to ~3000 reactions on a single highly multiplexed RT-PCR plate. We are currently accepting samples that have already been converted to cDNA for running on custom OpenArrays that have been printed by BioTrove. These arrays can be printed in individualized formats varying from 2 samples x 1344 reactions to 48 samples x 56 reactions (contact us for more information) using either SYBR Green or Taqman probes. Alternatively, users can purchase pathway specific OpenArrays directly from BioTrove which hold 4 samples with ~600 reactions per sample.

For more detailed information about the system and capabilities see:

*www.biotrove.com

For information about accessing the system including detailed pricing and availability please contact the Gladstone Genomics Core. Please see the Gladstone Genomics Core Price List for basic services and UCSF pricing.