Transgenic Mice
For a typical construct, 3—5 recipients are implanted with microinjected DNA supplied by the investigator. Litters of 6—8 pups are usually produced by 2/3 of the implanted recipients. The Core maintains a mating colony of C57BL/6 inbred mice, the most frequently requested strain for transgenic founders and the standard service of the Core. A mating colony of inbred FVB mice is also widely used and frequently available for service. The Core has also used C57BL/6 x SJL F1 hybrids and DBA inbred strains, and can establish a mating colony (subject to a setup recharge) for requested work.
Rederive Mouse Lines
Mice housed in conventional non-barrier facilities are sent to quarantine rooms in the Gladstone Animal Facility for rederivation into pathogen-free lines. Mating is done in a quarantine room, then embryos are harvested and transferred to the Core laboratory for implantation into pathogen-free recipient females.
In-vitro fertilization
This technique is typically used to recover valuable lines from older mice not capable of successful mating. The core has recovered lines from single inbred or hybrid male mice of more than one year of age through this technology. Recovery of lines from frozen sperm stored in liquid nitrogen may be possible with hybrid lines but may not be successful with inbred lines. The Core keeps track of new developments in this area, and interested investigators should consult with the Core.
Blastocyst implantation
The Transgenic Core performs provides blastocyst injection and implantation services for the Gladstone Institutes Blastocyst Core, supervised by Sylvaine Cases at the Gladstone Institutes. Investigators interested in generating gene-targeted mice should consult with the Blastocyst Core for help with construct design and stem cell preparation
Prices
Please contact us for pricing information:
Paul Swinton pswinton@gladstone.ucsf.edu
Sid Espineda sespineda@gladstone.ucsf.edu
